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KMID : 0380020040190050406
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 5 p.406 ~ p.409
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Identification of Key beta-1.3-glucan Synthesis Enzymes in Agrobacterium sp. ATCC31750
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Kim Li-Hua
Lee Jung-Heon
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Abstract
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Matrix Assisted Laser Desorption ionization Time of Flight (MALDI-TOF) was used for enzymes identification related to B -1,3-glucan synthesis. Agrobacterium sp. ATCC31750 was cultivated with two stage Continuous Stirrer Tank Reactor (CSTR) and the cells were harvested and their protein profiles were analysed by two dimensional electrophoresis. The specific enzyme spot was treated with trypsin and ana lysed by MALDI-TOF to get peptide molecular weight. The peptide molecular weights were matched with Agrobacterium tumefacience’s Data Base from the matrix science site, then could identify the avaliable key enzymes. In this study, we identified key metabolite of synthesis of beta-1,3-glucan, such as glucose-6-phosphate isomerase, phosphoglucomutase, B-1,3-glucan synthase and glucokinase, and we also identified uracil phosphoribocyl transferase and Ribosome recycling factor also.
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KEYWORD
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beta-1, 3-glucan(curdlan), MADI-TOF, 2D-SDS-PAGE
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